National Research Council of Italy

Institute of Biosciences and BioResources

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IBBR Project #155

Identification of micropredators of barren-forming sea urchins by molecular markers
Project ID 155
ID 000000
Acronym ACCORDI BILATERALI ITALIA MONTENEGRO
Project acronym and title Identification of micropredators of barren-forming sea urchins by molecular markers
Contract 000000
Description The project wi use modern molecular techniques to amplify trace DNA remains in the guts of small invertebrate, in order to identify potential micropredators of sea urchins. DNA analysis is a recent advancement in the study of predator-prey interaction and, to date, has never been used for sea urchins. Objectives of the present project are: 1) Design of specific and group primers for DNA from the two most common Mediterranean se urchin species from rocky reefs, Paracentrotus lividus and Arbacia lixula. This task will be facilitated by the presence of a wide collection of expressed sequence tags (ESTs) in public databases, eg. www.ncbi.nlm.nih.gov. For P. lividus, the complete genome sequence is already known and available. We will design primers based on both mitochondrial (cycloygenase I, ITS) and nuclear (bindin, P16, P19) DNA, known to be highly polymorphic among species 2) Identification of potential micropredators of sea urchin by amplification of total DNA with the specific primers designed in objective 1. PCR techniques will overcome the challenges of handling small invertebrates, stored in ethanol for weeks or months
Funding body ACCORDI BILATERALI
UOS Palermo
Role Partner
Signatory Vendramin Giovanni Giuseppe
Contact Person De Michele Roberto
Starting of activities 2015
Ending of activities 2016
Extension -
Amount (euro) 5000.00
Technological Area -
Technological Field Ottimizzazione dell’uso delle risorse naturali negli ecosistemi agricoli e forestali
Select Projects
Istituto di Bioscienze e Biorisorse (IBBR/CNR)
Via G. Amendola 165/A, I-70126 Bari (Italy)
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